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Zero blunt topo cloning manual transmission

Zero Blunt TOPO PCR Cloning Kit. Fiveminute cloning of bluntend PCR products. Catalog numbers K, K, K2800J10, K, K, K, Zero Blunt TOPO PCR Cloning Kits are available with One Shot supplied with Cat. no. K, refer to the manual supplied with the miniprep kit. TOPO II Zero Blunt PCR Cloning Kit [Notes: For bluntend cloning (NO TAoverhang, dont use Taqbased) of PCR fragments. Kit recommends not exceeding 3, 000 bp inserts (and dont use fragments that are too small either).

Invitrogen Zero Blunt TOPO PCR Cloning Kit, without competent cells Provides a highly efficient, 5minute, onestep cloning strategy ( in. TOPO Cloning in. ) for the direct insertion of bluntended PCR products amplified with proofreading thermostable polymerases into a plasmid vector Zero Blunt TOPO PCR Cloning Kit provides a highly efficient, 5minute, onestep cloning strategy (" TOPO Cloning" ) for the direct insertion of bluntended PCR products amplified with proofreading thermostable polymerases into a plasmid vector.

TOPO cloning is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement for DNA ligases. For TOPO TA cloning, the Taq polymerase has a nontemplatedependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3'end of the PCR products.

Invitrogen Zero Blunt TOPO PCR Cloning Kit, with pCRBlunt IITOPO Vector, One Shot TOP10 Chemically Competent E. coli, and PureLink Quick Plasmid Miniprep Kit Offers the fastest and easiest method for highefficiency (cloning of bluntend PCR products amplified with proofreading thermostable polymerases No matter what your downstream application is, TOPO PCR cloning solutions help you get the right clone sooner, freeing up your time to answer more important questions.

TOPO Tip of the Month Eliminate bluewhite screening with TOPO Zero Blunt vectors, which contain a lethal ccdB gene. Zero Blunt PCR Cloning Kit A high efficiency system for cloning bluntended PCR products Catalog numbers K, K, K, and K Document Part Number Publication Number MAN Type of kits This manual is supplied with the following kits.

Introduction Zero Blunt TOPO PCR Cloning provides a highly efficient, 5 minute, onestep Zero blunt topo cloning manual transmission strategy (" TOPO Cloning" ) for the direct insertion of bluntend PCR products into a plasmid vector.

Zero Blunt TOPO PCR Cloning Kits offers the fastest and easiest method for highefficiency (cloning of bluntend PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase Iactivated pCR Blunt IITOPO Vector for 5minute benchtop ligations without ligase (1).

At the moment I am trying to ligate a (pretty large) 3, 8kb fragment (Gelextraction purified in high yields) into a Zero Blunt TOPO vector using its original protocol. 5 Product information Contents and storage Shipping and Storage Zero Blunt TOPO PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells contain a box with Zero Blunt TOPO