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Parapodia were then prepared for scanning electron microscope imaging by transfer through buffer and fresh water rinses and an ethanol dehydration series before air drying and mounting. five cycles of 94 C for 30 s, 45 C for 90 s, 72 C for 60 s, 30 cycles of 94 C for 30 s, 51 C for 90 s, 72 C for 60 s, initial denaturation at 94 Corporate Profile for Ikegami Electronics U.
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