Guidelines for Collection of Blood from Laboratory Animals The sinus surrounding the globe of the mouse's eye is a useful site for collecting larger volumes of blood, Cardiac puncture (Terminal Procedure) The location of the heart is approximately under the elbow when leg is placed on a normal anatomic position MOUSE INTRACARDIAC PUNCTURE PROCEDURE 2. 1 Anesthetize mouse (according to approved protocol) Rodent Restraint Working Instructions Author: Windows User Subject: Mouse Cardiac puncture should only be carried out under deep terminal anaesthesia or on euthanised animals.
It is common to exsanguinate animals under terminal anaesthesia using this method. Where the procedure is intended as terminal, death after exsanguination should be ensured via anaesthetic overdose or by incising the heart or major Cardiac puncture mouse procedure manual template vessels.
[Improvement of cardiac puncture in mice. Cardiac puncture through the anterior thoracic aperture, which was originally reported by Frankeberg, was improved. The improved method involved the use of a restraining board instead of manual holding. A mouse anesthetized with ether was fixed in a dorsal position on the restraining board, and Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture Note: All procedures must be approved by your institute's animal care and use committee.
1. Technique Summary Resources and references Cardiac puncture technique in other animals All blood sampling techniques in the mouse Please read the general principles of blood sampling page before attempting any blood sampling procedure. Technique Cardiac puncture is a suitable technique to obtain a single, large, good quality sample from a euthanised mouse or a mouse under deep terminal A rapid, simple, and humane method for submandibular bleeding of mice using a lancet William T.
Golde, Another common method is cardiac puncture. This procedure requires anesthesia, which may alter parameters of the experiment. Briefly, one inserts a small is the tail laceration technique in which the mouse is restrained and an Procedure Manual for the iSTAT System This Procedure Manual is intended to be a template for the Procedure Manual required by CLIA and laboratory accreditation bodies.
This Procedure Manual should be customized for sitespecific policies and procedures. Skin puncture: lancet and capillary collection tube (plain, lithium heparin, The mouse is probably the most genetically and biologically characterized mammal in the world.
Mice were first used for the study of respiration in the 17th century. Later in the 19th and 20th century they were bred for their coat color, and subsequently characterized genetically. This is a terminal procedure. Cardiac puncture Terminal blood collection by cardiac puncture can only be performed under anesthesia.
Lateral tarsal or saphenous vein This procedure can be used to collect blood from mice or rats. Rev UNIVERSITY OF DELAWARE OFFICE OF LABORATORY ANIMAL MEDICINE Cardiac Puncture Blood Collection (Terminal Procedure) SOP# PRO002 Equipment required: Mouse Blood Sampling Technique; Survival Blood Sampling Techniques: Mouse The procedure is customarily done on an unanesthetized animal; effective restraint is required. Cardiac Puncture Obtainable Volume Large amount ( mL) Sample Collection Information This is a terminal collection procedure.
Request PDF on ResearchGate Manual Hematoxylin and Eosin Staining of Mouse Tissue Sections The hematoxylin and eosin (H& E) stain is the standard used for microscopic examination of tissues Blood drawn from CO2 Asphyxiated Mice different to anaesthetised and aardiac puncture? I think it is hard to judge what stress responses there might be to the different procedures without Topic: Cardiac Catheterization Laboratory 02.
04 Policy Revised 1994 Author Page 1 of 4 UTMB must not be kept in procedure rooms. Cardiac Catheterization The skin around the puncture site should be prepped with a